This position is in the laboratory of Ellen Gravallese, MD, Chief of the Division of Rheumatology, Inflammation, and Immunity. Our group is studying inflammation and osteoimmunology in the context of autoimmune diseases such as rheumatoid arthritis and spondylarthritis. In addition, we study innate immune mechanisms of autoimmune and autoinflammatory diseases. We utilize animal models of disease, human tissue samples, and in vitro experiments to study the pathways by which inflammation is mediated and also by which bone homeostasis is disrupted in disease states.
We are looking for a motivated and organized individual with hands-on experience working in the laboratory. The person filling this position will work closely with senior members of the lab, supporting them in various aspects of their ongoing research projects. In addition, it would be ideal if this candidate could also assist in our animal facility. The ideal candidate will have experience performing ELISAs, Westerns, primary cell culture, qPCR and histology. Experience in animal husbandry, genotyping (PCR), dissection and tissue harvesting, and organization of the animal database is also desirable, but not required. Finally, experience with analysis of bulk and single cell RNA sequencing data and the use of R would be an advantage.
Principal Duties and Responsibilities:
- Bench work
- RT-PCR/qPCR
- ELISA
- Primary cell culture
- RNA extraction
- Westerns
- Microscopy
- FACs analysis
- Sample preparation for RNA sequencing
- Histological techniques: Immunohistochemistry, Immunofluorescence
- Working knowledge of databases/spreadsheets software (Microsoft Office)
- Maintenance of mouse colony, including weaning, ear tagging, genotyping by PCR, setting up breeding and record keeping.
Additional Duties/Skills/Competencies:
- Keeping an up to date laboratory notebook
- Assisting in maintaining lab space, equipment, and supplies
- Willingness to learn new laboratory techniques
- Ability to communicate well, both verbally and in writing
- Good organizational skills
- Ability to work closely with the research team
- Self-motivated and capable of troubleshooting and creative problem solving
Qualifications - External:
- Bachelor's degree or equivalent required (suitable field preferred).
- Relevant biology course work is preferred.
- Demonstrated organizational and interpersonal skills as required to work closely with a research team.
- Demonstrated competence with databases/spreadsheets and software packages.
EEO Statement:
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
Primary Location: MA-Boston-BWH Longwood Medical Area
Work Locations:
BWH Longwood Medical Area
75 Francis Street
Boston 02115
Job: Technician/Technologist/Scientist
Organization: Brigham & Women's Hospital(BWH)
Schedule: Full-time
Standard Hours: 40
Shift: Day Job
Employee Status: Regular
Recruiting Department: BWH DOM Rheumatology
Job Posting: Sep 23, 2024
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