About Conifer Life Sciences Group, LLC
Conifer Life Sciences Group, LLC was established to ideate, develop, and launch new ventures in the life science tools industry. A Conifer portfolio company, currently in stealth mode, has an opening for a Research Associate.
This is an excellent opportunity for a motivated Research Associate to join a company on the ground floor. We aim to produce the best quality UPS protein reagents, assays, and custom services. Your contributions will have an immediate impact. We are seeking collaborative, relentless problem solvers who share our passion for making an impact.
Position:
We seek a motivated and highly skilled Research Associate to perform research experiments as defined by the supervisor and provide operational support to the laboratory. The ideal applicant will be a motivated individual with experience in protein purification and in vitro assay development.
Key Responsibilities :
· Use existing protocols to purify recombinant proteins to provide to the UPS community.
· Support scientists in bacterial transformations, expression analysis, large-scale harvest, large-scale purification, and analytical characterization of protein products.
· Develop new purification protocols using chromatographic methods, including affinity, ion exchange, SEC Chromatography, HIC, etc.
· Characterize protein purity and activity using analytical SEC, DSF, and qualitative enzymatic assays.
· Develop expression, purification, and labeling methods for custom projects and new catalog products. Characterize enzymatic activity using a combination of qualitative and quantitative assays for analysis
· Learn and help develop in vitro assays using Alpha, HTRF, FP, and luminescence techniques
· Perform additional job-related duties as required, such as reagent preparation, ordering, and maintaining an ELN
· Other duties as assigned.
- Qualifications : BS or MS in Biology, Biochemistry, or equivalent with a minimum of 2 years of laboratory experience in academic or industrial settings.
- Experience using techniques such as buffer preparation, bacterial fermentation using shaker flasks, SDS-PAGE, ELISA and Western Blot analysis.
· A strong understanding of chromatography techniques, including ion exchange and size exclusion. Experience using FPLC or HPLC systems is a plus.
· Some lab experience in IEX, HIC, AKTA purification, SEC-HPLC, SDS-PAGE, UV/Vis spectrophotometry
· Experience with aseptic techniques and bacterial cultures.
- A strong work ethic within a team environment.
· Basic understanding of lab safety and protocols (GLP)
- Knowledge of Microsoft Office products, including Excel
- This is a full-time, on-site role.
Conifer Life Sciences Group, LLC is an Equal Opportunity employer and does not discriminate against an applicant or employee because of race, creed, color, religion, sex, national origin, ancestry, age, sexual orientation, p
physical or mental disability, or veteran status.
The duties listed in this job description are intended only as illustrations of the various types of work that may be performed. The omission of specific statements of duties does not exclude them from the position if the work is similar, related, or a logical assignment to the position.